A Field Manual for Community Dengue Response · Pune District, Maharashtra

Don't Let
It Fester

When externally funded global health programmes contract, the dengue burden does not — it shifts onto frontline community workers. This manual consolidates surveillance, prevention, and reporting guidance for ASHA workers and NGO staff already doing that work in Pune District.

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Built specifically for Pune District, Maharashtra — with local data, Marathi-language resources, and the PMC/NCVBDC reporting chain. Designed as a replicable template: see the localisation guide.

13%Projected rise in dengue mortality in Pune District by 2040 — reaching 23–40% by 2060 (Murtugadde et al., Nature 2025)
100Rapid antigen tests per year at Karanjawane PHC in Pune District — the documented resource gap (Gavi, 2025)
87%Estimated cut to global epidemic & disease surveillance funding since January 2025 (Cavalcanti et al., The Lancet 2025)

What's Inside

Six sections, organised around what a community health practitioner in Pune District actually does. This site consolidates and synthesises existing guidance — it does not replace official protocol or local training.

Why this manual exists

The Infrastructure Gap

Dengue in urban Pune is both a disease of poverty and a failure of governance. The 2025 contraction of Western-funded global health infrastructure did not make the disease go away. It displaced the work of responding onto community actors who are already under-equipped and over-stretched.

01 — Threat

A rising threat in Pune

Climate change is making Pune one of India's leading hotspot for dengue outbreaks — dengue mortality is projected to rise 13% by 2040, 23–40% by 2060, and up to 112% by 2100. More than 80% of dengue deaths in Pune District occur after monsoon onset in June.

02 — Shock

An 87% funding shock

Since January 2025, USAID-supported NGOs, technical units, and surveillance partnerships in India lost funding abruptly. WHO reported over 70% of surveyed country offices saw service disruptions, with vector-borne disease programmes among the hardest hit.

03 — Inequity

An unequal burden in Pune

Dengue concentrates in Pune's lower-income informal settlements where intermittent municipal supply forces water storage. Wealthier housing societies in Pune often resist PMC vector control entry. Pune tops Maharashtra's district rankings for dengue-related mortality.

📍 Pune District — Karanjawane PHC

In Karanjawane village in Pune District, the ASHA team at the Primary Health Centre is already doing this work. ASHA worker Manisha Ratan Nidhalkar, with over a decade of experience, teaches residents to identify Aedes larvae on sight — with a torch, because phone lights are insufficient. Community awareness has reached the point that regular citizens can spot larvae themselves.

But the resource gap is stark. Laboratory Scientific Officer Bharti Mali at the same PHC notes that her facility receives only 100 rapid antigen tests per year from the local government. As externally funded NGO infrastructure disappears, this community capacity exists — but is operating without the tools and structured support it needs.

Source: Sweta Daga, "Dengue Risk in India Is Rising," Gavi/VaccinesWork, 6 May 2025
Both in drought and in rains, we have an issue with mosquitoes. When it's dry, people store water — that breeds mosquitoes. When the monsoon arrives and rains pour, puddles stay for days — that breeds mosquitoes too. Srikanth Darwatkar, SATHI (Support for Advocacy and Training to Health Initiatives), Pune — Gavi, 2025

Section 02

Community Surveillance

Surveillance is the early-warning system. Household larval surveys let community workers detect mosquito breeding before cases reach the clinic. These three indices are the standard measures used in NCVBDC vector-borne disease programmes — they turn a walk through a Pune neighbourhood into data a Primary Health Centre can act on.

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Pune District: When to intensify surveillance

Over 80% of dengue deaths in Pune District occur after monsoon onset in June. Intensify household surveys from June through November. SATHI field data from Pune confirms the double-bind: water storage during dry months (often March–May) and monsoon puddles (June–October) both sustain breeding year-round.

Mar Apr May Jun ▲ Jul ▲ Aug ▲ Sep ▲ Oct ▲ Nov Dec

The three larval indices

Record these during routine household visits. Together they answer: how widespread is breeding, and how intense?

HI

House Index

Percentage of houses inspected with at least one container positive for larvae or pupae.

CI

Container Index

Percentage of all water-holding containers inspected that are positive for larvae or pupae.

BI

Breteau Index

Number of positive containers per 100 houses — the most informative single measure for outbreak risk.

NCVBDC Risk Thresholds — confirm current values with your Pune District VBD Officer before each monsoon season

BI < 5
Low Risk
Routine surveillance; maintain dry-day routine
BI 5–20
Moderate Risk
Intensify source reduction; alert PHC; increase visit frequency
BI > 20
High Risk / Alert
Immediate escalation to PHC and District Surveillance Officer; trigger outbreak response
Verify: Confirm these thresholds with the Pune District VBD Officer, District Health Office, Pune and the NCVBDC Integrated Vector Management guidelines before the monsoon season. Thresholds are reviewed annually. NIV Pune tests suspected samples directly — contact: niv.icmr.org.in, 20-A Dr. Ambedkar Road, Pune 411001.

Running a household larval survey in Pune

A repeatable routine any trained ASHA can carry out. Key Pune-specific adaptations below each step.

  1. Map the cluster and note water supply days.

    Define the houses to be covered and note which days the municipal supply runs in each area. Pune informal settlements often receive water 3–5 days per week — surveys on the day after a supply gap often find more stored water and more breeding sites.

  2. Inspect every water source — use a torch, not a phone light.

    Check all containers holding water, indoors and outdoors. Aedes larvae sit just below the surface and dart down when disturbed. Phone lights are not bright enough to see larvae in dark containers — Karanjawane ASHA teams confirmed this and always carry a dedicated torch.

  3. Record positives by container type.

    Note each container type (drum, overhead tank, cooler, flower pot) positive for larvae or pupae. Tally houses and containers for the three indices. In Pune informal settlements, overhead water storage tanks and plastic drums are the most common positive sites.

  4. Act on the spot.

    Empty or treat positive containers immediately where possible. Explain to the household why — in Marathi, using familiar language about children's health rather than technical terminology.

  5. Report upward to PHC on the agreed schedule.

    Submit index data to the supervising Primary Health Centre. In Pune District, suspected samples are tested directly at NIV Pune — the PHC Medical Officer coordinates with the District Surveillance Officer for formal outbreak reporting via IHIP.

📍 Pune Slum Research Finding

A cross-sectional study of five Pune slums (Savitribai Phule Pune University, 2017–18, n=309) found that while 81% of residents knew to prevent standing water, only 26.1% knew about indoor breeding sites and only 38.1% knew outdoor breeding sites. This gap between general awareness and specific action knowledge is the exact thing ASHA household visits close. The study found literacy was strongly associated with knowledge — reinforcing the value of in-person demonstration over written materials alone.

Source: Savitribai Phule Pune University community study published via medRxiv, 2020

Section 03

Finding & Removing Breeding Sites

Aedes aegypti breeds in clean, still water close to homes and bites mainly during daytime hours — 6–9am and 4–6pm are peak biting periods in Pune. In urban informal settlements where PMC supply is intermittent, stored water is unavoidable and a daily survival strategy. Source reduction, not blame, is the goal.

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Pune-specific context: The water storage dilemma

Pune's informal settlements receive municipal water 3–5 days per week on average. Residents have no choice but to store water in drums, pots, and tanks — this is not negligence, it is adaptation to intermittent supply. Effective source reduction works with this reality: covering, treating, and scrubbing regularly rather than eliminating storage containers that families depend on. SATHI's Pune field work documents that messaging that ignores this double-bind immediately loses community trust.

Common breeding sites in Pune's urban informal settlements

If it holds water for more than a few days, check it. In Pune, PMC field surveys consistently find overhead and ground-level storage tanks as the highest-positivity container type.

  • Overhead & ground-level plastic water storage tanks (most common positive site in Pune surveys)
  • 50–200 litre drums and barrels for drinking water storage
  • Desert air coolers — check and empty weekly during off-season storage
  • Discarded tyres, tins, plastic containers in open lots
  • Flower pots, saucers & money-plant water bottles indoors
  • Refrigerator and AC defrost trays
  • Construction-site pits and curing water (major source in Pune's rapid-build areas)
  • Blocked roof gutters and junk collecting rainwater
  • Coconut shells, cut bottles, rain-catching litter
  • Pet and bird water containers — change water every 2 days
  • Roadside puddles and low-lying open drains after monsoon rains
  • Terrace gardens and planters in multi-storey buildings

Source reduction methods — matched to Pune context

The PMC-endorsed Dry Day routine is the core prevention practice. Align with the city campaign and reinforce it in every household visit.

  1. PMC Dry Day — empty, scrub, and dry all containers once a week.

    PMC runs anti-dengue Dry Day campaigns across Pune city. Community workers should reinforce the same message: it takes 7–10 days for the mosquito life cycle to complete, so weekly scrubbing breaks the cycle. Scrubbing matters because Aedes eggs cling to container walls and survive drying — just emptying without scrubbing is not enough.

  2. Cover what cannot be emptied.

    Tightly cover storage tanks and large drums with lids or mesh so mosquitoes cannot access the water surface to lay eggs. PMC has issued mesh covers in some areas — check what is currently available from your ward-level PMC health office.

  3. Treat large fixed tanks with approved larvicide.

    For overhead tanks that cannot be emptied, temephos (Abate) larvicide is the NCVBDC-approved treatment. This should only be applied under guidance from the PHC or District VBD programme. Do not use unapproved products.

  4. Address construction sites — report to PMC.

    Construction activity is a major driver of dengue in Pune's fast-expanding periphery. Stagnant water in construction pits is a high-yield breeding site. ASHAs can report active construction sites with uncovered water accumulation to the PMC Health Department for formal notice issuance.

  5. Community clean-up drives.

    Organise mohalla-level drives targeting tyres, litter, and open drains — especially in the two weeks before monsoon onset (late May/early June in Pune). Mahila Arogya Samitis are the most effective organising unit for this in Pune informal settlements.

PMC Contact for larvicide and source reduction support: Pune Municipal Corporation Health Department, Jumbo COVID Centre (now repurposed as Health HQ), Pune Camp Area. For official IEC materials, contact the PMC Health Department: pmc.gov.in

Section 04

Talking to Communities

Surveillance and source reduction only work if households cooperate. Effective communication in Pune is in Marathi, delivered by trusted neighbours, and names the real constraints people face — especially intermittent water supply — rather than pretending they don't exist. Research from Pune slums shows the gap between awareness and action is not a knowledge problem; it is a trust and resources problem.

✓ What works — Pune-specific

  • Use Marathi as the default language — ASHAs reach households far more effectively in Marathi than Hindi or English; PMC IEC materials exist in Marathi and should be the first choicePune slum study, Savitribai Phule Pune University, 2020
  • Teach larvae identification by sight using a torch — demonstrate in person inside the home; Karanjawane ASHA teams already do this with documented successGavi/Sweta Daga, 2025
  • Invoke SATHI's framing: stored water is a survival response to intermittent supply — name this explicitly so residents understand the message is about management, not blameSATHI/Darwatkar, Gavi 2025
  • Work through Mahila Arogya Samitis (women's collectives) as trusted local messengers alongside ASHA workers — these are Pune's most effective community mobilisation unit for sustained behaviour change
  • Connect the Dry Day routine to school calendars — families in Pune informal settlements already organise heavily around school-age children's health; linking prevention to children's well-being lands far more effectively than disease statistics
  • Return regularly — outbreak-driven visits that disappear between seasons are one of the documented failures in Pune; continuous relationship is what converts awareness into sustained practice

✕ What backfires — documented in Pune

  • Blaming residents for storing water when PMC supply is intermittent 3–5 days per week in many Pune informal settlements — this destroys trust immediately and is the single most common communication failureSATHI field observation; Gavi 2025
  • Assuming prior knowledge of indoor breeding sites — Pune slum study found only 26% of residents knew about indoor breeding locations; messages that skip this step miss most of their audienceSavitribai Phule Pune Univ study, 2020
  • One-off outbreak-driven campaigns without follow-up — Pune data shows awareness spikes during outbreaks but does not translate to sustained behaviour change without ongoing engagementMishra et al., Frontiers Public Health 2024
  • Fines-first enforcement without education — PMC issued 1,000+ notices and levied ₹3.87 lakh in penalties in 2024; this punitive approach, applied disproportionately to informal settlements, alienates the communities that most need supportBusiness Standard/PMC Health Dept 2024
  • Messaging in Hindi or English only in areas where Marathi is the primary household language — creates distance and signals the message is not really for this community
  • Treating wealthier and poorer neighbourhoods unequally and then accepting that gap as normal — research documents that dengue in Delhi concentrates in lower-income areas precisely because vector control is enforced there and resisted in wealthier areasSingh & Chaturvedi, Scientific Reports 2024
📍 The politics of enforcement — what this toolkit names directly

In Pune, as in other Indian cities, dengue surveillance and enforcement are applied unequally. PMC notices go predominantly to construction sites and informal settlements. Wealthier residential societies — which may have significant rooftop water storage, terrace gardens, and AC systems — are often able to resist municipal vector control entry. This is not incidental to the disease burden; it is structural. ASHA workers and NGO staff should be aware that their communities bear a disproportionate enforcement burden alongside a disproportionate disease burden.

The goal of community communication is not compliance enforcement — it is shared ownership of a shared problem. Framing prevention as something the community is doing for its own protection, rather than something being done to it by the municipality, consistently produces better and more sustained results in field settings across urban India.

Marathi-language IEC materials available from PMC

Use existing materials rather than designing from scratch. PMC Health Department has produced the following resources in Marathi — confirm current availability at your ward-level PMC office:

  • डेंगू प्रतिबंधक जनजागृती माहितीपत्रक (Dengue prevention awareness leaflet — PMC)
  • कोरडा दिवस मोहीम सूचनापत्र (Dry Day campaign notice)
  • डास उत्पत्ती स्थान ओळख (Mosquito breeding site identification poster)
  • NVBDCP IEC Materials in Marathi — available via Maharashtra State Health Dept
  • PMC Anti-Dengue Campaign social media cards (shareable via WhatsApp)
  • ASHA Diary/Log Book — available from your ANM/LHV supervisor
Request materials from: Your ward-level PMC Sub-Health Centre or the PMC Health Department, Pune. NVBDCP national IEC resources (available in multiple languages including Marathi) can be accessed via: ncvbdc.mohfw.gov.in

Section 05

Reporting & Escalation

Dengue is a notifiable disease in India under the Integrated Disease Surveillance Programme (IDSP). Timely reporting connects a single ASHA's observation in Pune to a coordinated district-level response. As externally-funded technical units disappear, the official government reporting chain becomes more important — it does not require donor funding to function.

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When to escalate — the core signals

Do not wait for confirmed cases before reporting. A cluster of fever cases in one area, larval indices rising above the Moderate threshold (BI > 5), or any suspected severe dengue case (bleeding, persistent vomiting, rapid breathing) are signals to escalate immediately. In Pune, suspected dengue samples are tested at NIV Pune — the fastest diagnostic route in the district.

The Pune District reporting chain — step by step

Follow this chain in sequence. Each step has a formal role responsible for the next action.

  1. ASHA / Community Worker
    Identify the signal and document it
    Record: number of suspected fever cases, cluster location, larval index data from most recent household survey. Use the S-Form (Syndromic Surveillance) for suspected cases — your ANM/LHV supervisor has these forms or they can be entered directly into the IHIP mobile app (available on Android). Do not diagnose — document and report.
  2. Primary Health Centre (PHC)
    PHC Medical Officer — first formal response point
    Pass your S-Form data and larval survey results to the PHC Medical Officer. In Pune rural areas, PHCs are the first formal surveillance node. For Pune City (PMC limits), report to your ward-level PMC Sub-Health Centre Medical Officer. The PHC/SHC submits weekly surveillance data to the District Surveillance Unit via IHIP.
  3. NIV Pune — Sample Testing
    National Institute of Virology, Pune — diagnostic confirmation
    Suspected dengue samples in Pune District are tested directly at NIV Pune (ICMR) — the apex national virology lab, located at 20-A Dr. Ambedkar Road, Pune 411001. The PHC Medical Officer coordinates sample dispatch. NS1 antigen and IgM ELISA are both required for complete diagnosis — using only one test misses a significant proportion of cases.
  4. District Surveillance Unit
    District Surveillance Officer — outbreak investigation trigger
    The District Surveillance Officer (DSO), Pune receives compiled IHIP data from PHCs and investigates signals of outbreak. The DSO coordinates with the District Malaria Officer (DMO) — currently Dr. Aparna Patil at the District Health Office, Pune — for vector-borne disease response including fogging, intensified source reduction, and additional larvicide deployment.
  5. State / National Level
    Maharashtra State IDSP Unit → NCVBDC → Central Surveillance Unit
    District data feeds into the Maharashtra State Surveillance Unit and then into the National IHIP platform (ihip.nhp.gov.in), which monitors for outbreak signals across India in near-real-time. The NCVBDC Central Surveillance Unit at NCDC, Delhi, coordinates the national response. For urgent outbreak notification: idsp-npo@nic.in, +91-11-23932290.
📍 Pune-specific: The NIV testing advantage

Pune District has a significant advantage that most of India does not: the National Institute of Virology (NIV), India's premier virology research institute, is based in Pune. Suspected dengue samples in Pune District are tested at NIV directly, which provides faster and more comprehensive serotyping than most district-level labs can offer. The Frontiers in Public Health (2024) Pune study found that using NS1 alone missed 43% of confirmed dengue cases, and IgM alone missed 25.5% — both tests should be used together. When advocating with your PHC for adequate testing, this research supports requesting both tests for suspected cases.

Source: Mishra et al., "Dengue in Pune city, India (2017–2019)," Frontiers in Public Health, September 2024
IDSP/IHIP mobile app for field reporting: The IHIP Android app (available on Play Store: search "IHIP IDSP") allows ASHA workers to submit S-Form data in real time from the field. Your ANM or PHC data manager can set up access. This removes the delay of paper-based reporting and allows the District Surveillance Unit to see emerging clusters faster.

Before you begin

How to Use This Manual

This site consolidates and synthesises existing guidance for Pune District. It is a companion to official protocol and local training, never a replacement for them. It is designed as a replicable template — the localisation guide below explains how to adapt it for another district.

A starting point

Always follow your district health authority's current instructions where they differ from what is here. Official NCVBDC and PMC protocol takes precedence over anything in this site.

Meant to be localised

Larval index thresholds, reporting contacts, and IEC language vary by district. This toolkit is built for Pune — see the localisation guide in Section 06 if you are adapting it for another district.

Built for low resources

Every method here is designed to work without external donor funding — using the public system, community capacity, and government protocol that remain even as NGO infrastructure contracts.

Section 06

Official Resources & Links

This section points to the authoritative sources for Pune District practitioners — government guidelines, reporting systems, and the local organisations you can actually contact.

Government of India

National Centre for Vector Borne Diseases Control (NCVBDC)

The apex body for dengue control in India. Integrated Vector Management guidelines, larval survey protocols, and the National Dengue Clinical Management Guidelines (2023 edition).

→ ncvbdc.mohfw.gov.in

IEC materials available in Marathi — request via Maharashtra State VBD Programme or download from NCVBDC portal

Government of India / Maharashtra

Integrated Disease Surveillance Programme — IHIP

The national outbreak-detection system that all PHC surveillance data feeds into. The IHIP mobile app enables ASHA-level real-time reporting. S-Form, P-Form, and L-Form templates available.

→ ihip.nhp.gov.in
→ idsp.mohfw.gov.in
Pune Municipal Corporation

PMC Health Department — Dengue & Vector Control

Pune-specific anti-dengue campaigns, Dry Day initiative materials, IEC in Marathi, and the ward-level Health Officer contact network. PMC has issued enforcement notices and anti-mosquito campaigns since 2024.

→ pmc.gov.in/en/health

Marathi IEC materials: request from your ward PMC Sub-Health Centre

ICMR — National Institute of Virology

NIV Pune — Dengue Diagnostics

India's premier virology lab, based in Pune. Suspected dengue samples in Pune District are tested at NIV. PHC Medical Officers coordinate sample dispatch. Both NS1 and IgM tests should be requested for suspected cases.

→ niv.icmr.org.in
20-A Dr. Ambedkar Road, Pune 411001
Pune-based NGO

SATHI — Support for Advocacy and Training to Health Initiatives

Pune-based health organisation that supports public health systems in Maharashtra. SATHI's field workers document the water-storage double-bind in Pune informal settlements and provide community health training support.

→ sathicehat.org
World Health Organization

WHO Dengue Guidance

Global technical guidance on dengue surveillance, vector management, and clinical case definitions. The WHO Global Vector Control Response 2017–2030 is the framework that the NCVBDC national programme aligns with.

→ WHO Dengue Fact Sheet
🗺️ How to localise this toolkit for another district

This site is a replicable template. If you are adapting it for a district other than Pune, here is what to replace and where to find it:

1. Larval index thresholds: Confirm BI/HI alert thresholds with your district VBD Officer or from your state's NCVBDC operational manual — these are district-specific and reviewed annually.

2. Reporting contacts: Replace NIV Pune with your district's designated diagnostic laboratory. Replace the District Malaria Officer name with the current officer from your District Health Office.

3. Language: Replace Marathi with the primary language(s) of your district. Check what IEC materials the State Health Department has produced in your language.

4. Water supply context: Investigate the specific intermittency pattern in your district's informal settlements — this shapes which container types are highest risk and how to frame the conversation about water storage.

5. Local NGOs: Replace SATHI with the community health organisations operating in your district — district-level NGO networks can usually be identified via your State NRHM office.

This localisation guide responds to the professor's observation that depth of a worked example (Pune) and reach of a generalizable model can coexist — Project Proposal, GLB HLT 191, Spring 2026

Background & Evidence Base

  1. Cavalcanti, D. M., et al. "Evaluating the Impact of Two Decades of USAID Interventions and Projecting the Effects of Defunding on Mortality up to 2030." The Lancet, 2025. https://doi.org/10.1016/S0140-6736(25)01186-9
  2. Daga, S. "Dengue Risk in India Is Rising. Hard-Hit Communities like This One Are Bracing for Impact." Gavi/VaccinesWork, 6 May 2025. [Karanjawane PHC, Pune District]
  3. Mishra, A. C., et al. "Dengue in Pune city, India (2017–2019): A Comprehensive Analysis." Frontiers in Public Health, vol. 12, September 2024. https://doi.org/10.3389/fpubh.2024.1354510
  4. Murtugadde, R., et al. "Dengue Dynamics, Predictions, and Future Increase under Changing Monsoon Climate in India." Scientific Reports, January 2025. [+13% Pune mortality projection]
  5. Singh, P. S., and Chaturvedi, H. K. "Socio-Ecological Predictors of Dengue in High Incidence Area of Delhi, India." Scientific Reports, vol. 14, no. 1, 23 July 2024. https://doi.org/10.1038/s41598-024-67909-7
  6. Savitribai Phule Pune University (SPPU). "Knowledge and Awareness of Dengue and Chikungunya amidst Recurrent Outbreaks amongst Urban Slum Community Members of Pune, India." medRxiv, 2020. [5-slum cross-sectional study, n=309]
  7. Shet, A., and Kang, G. "Dengue in India: Towards a Better Understanding of Priorities and Progress." International Journal of Infectious Diseases, vol. 84, July 2019, pp. S1–S3.
  8. World Health Organization. "Neglected Tropical Diseases Further Neglected due to ODA Cuts." WHO, 4 June 2025.
  9. Patil, A. (District Malaria Officer, Pune). Quoted in "Over 14,000 Dengue Cases in Maharashtra; Pune Sees Rising Trend." Free Press Journal, March 2026.